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Cap64 is required for the vesicle acidification and is localized at the site of quinacrine accumulation. The Cap64 was tagged with mCherry at its N terminus and the resulting strains were cultured in YPD liquid medium at 25 °C for 20 h. The subcellular localization of mCherry of Cap64 (a), quinacrine accumulation (b), the merged image (c), and DAPI morphology (d) were visualized under a Leica microscope and DIC (d−f). The scale bar shows 20 µm.
