Table 2.

Primers used for PCR in this study

Primer name	Sequence (5′−3′)
CAP64 primers for the qRT-PCR analysis
CAP64_F	CTGATCAAACCGATCTCGTCATTCT
CAP64_R	GATCAGGTCTCACAAGGATGCTTCT
LAC1 primers for the qRT-PCR analysis
RT-lac1.f2	AACGGTACTCTGCAATTATCAACA
RT-lac1.r2	AACCATTCCATTACCCGTATACCT
SAV1 primers for the qRT-PCR analysis
SAV1_RT_F	ATCTGTTACGATTGAGCAAGGA
SAV1_RT_R	AATCGCTTTGCTTGTTGACATT
VPH primers for the qRT-PCR analysis
VPH_RT_F	TGCCTCGATAAGTCCTGTAGAATT
VPH_RT_R	ATTACATTCCCATTCTTGTTTGCA
ACT1 primers for the qRT-PCR analysis
RT-ACT1.UP	ATGGAAGAAGAAGTCGCCGC
RT-ACT1.LP	TAGAAGGGAAGACAGCTCGG
mCherry primers for the qRT-PCR analysis
mcherry_RT_R	CGAGTTCATCTACAAGGTGAAGCT
mcherry_RT_F	TACATGAACTGAGGGGACAGGA
Primers for construction of the cassette coding CAP64 tagged with mCherry at the N-terminus of CAP64 (pYI602)
ter-cap64	TACTGTAACCCCATACTAGGTCCCAAAGCTACAGT
link-mecherry-R	GGCTTCTCTGAGCATCTTGTACAGCTCGTCCATGC
cap64-mcherry	GACGAGCTGTACAAGATGCTCAGAGAAGCCAAGGT
link-mecherry-F	TTAGCATAAATACAAATGGTGAGCAAGGGCGAGGA
CAP64-vector	AGCTTTGGGACCTAGTATGGGGTTACAGTAGCAGT
mche-vector	GCCCTTGCTCACCATTTGTATTTATGCtAAGTATA
Primers for construction of the cassette coding CAP64 tagged with mCherry at the C-terminus of CAP64 (pYI602) and for conforming transformants containing the cassette
mcherry-F	TGTAGCTTTGGGACCATGGTGAGCAAGGGCGAGGA
mcherry-R	CTACTGTAACCCCATTTACTTGTACAGCTCGTCCA
cap64-linker-F2	GCCCTTGCTCACCATGGTCCCAAAGCTACAGTCGT
cap64-linker-R2	GAGCTGTACAAGTAAATGGGGTTACAGTAGCAGTA
pro-cap64	TTAGCATAAATACAAATGCTCAGAGAAGCCAAGGT
Primers for amplification of the cassette
M13 forward primer	CGCCAGGGTTTTCCCAGTCACGAC
M13 reverse primer	AGCGGATAACAATTTCACACAGG