Table 1.

Primer sequences used for real-time polymerase chain reaction.

Gene	Forward (5’ → 3′)	Reverse (5’ → 3′)
COL2a1	CAA CAC TGC CAA CGT CCA GAT	TCT TGC AGT GGT AGG TGA TGT TCT
Aggrecan	ACA GCT GGG GAC ATT AGT GG	GTG GAA TGC AGA GGT GGT TT
COL10a1	AGC CAG GGT TGC CAG GAC CA	TTT TCC CAC TCC AGGAGGGC
PTHrP	ATG CAG CGG AGA CTG GTT CAG CAG T	GGG AGA GGG CTT GGA GTT AGG GG
Ihh	ATG TGC TCA TTT TCC TGG AC	CCA AGC TGT GAA AGA CTC TC
MMP13	TCT TGA GCT GGA CTC ATT GT	AGC CTC TCA GTC ATG GAG CT
Runx2 PTGS2	AGA TGG GAC TGT GGT TAC TG TGA GCA TCT ACG GTT TGC TG	GTA GCT ACT TGG GGA GGA TT TGC TTG TCT GGA ACA ACT GC
GAPDH	TCT CCT CTG ACT TCA ACA GCG AC	CCC TGT TGC TGT AGC CAA ATT C

Cycling conditions: Denature: 95 ​°C for 30 ​s, 95 ​°C for 4 ​min, followed by 35 cycles of 95 ​°C for 10 ​s, 55–61 ​°C(shown in column of Annealing Temperature) for 15 ​s and 72 ​°C for 15 ​s