Fig. 3.

Imaging of endosperms transfected with 35S::HDEL-mCherry and 35S::Rtn1-GFP. A, B) Endosperms bombarded using either 650 psi (A) or 1100 psi (B) rupture disks and imaged under a fluorescence dissecting microscope.? Rupture disk excited by light generated by a mercury lamp passing a DS-Red filter and a GFP filter, respectively. C,D) Confocal imaging and fluorescence spectral analysis of an endosperm epidermal/developing aleurone cell with positive mCherry-HDEL (C) and Rtn1-GFP (D) signals. The fluorescence spectral data was collected from the area indicated by yellow arrow.