Table 1.

Stock solutions for making the EICS culture medium supplemented with Carbenicillin.

Chemical	Concentration	Solvent	Sterilization	Amount to add to 250ml EICS medium after autoclave
MS powdered vitamin	1000x (as directed by the manufacturer)	Milli-Q water	filtration	1.25 ml
6-benzylaminopurine	5 µg/ml	Dimethyl sulfoxide	Not required	\
Thiamine HCl	25 mg/ml	Milli-Q water	filtration	50 µl
L-Asparagine (Asn)	40 mg/ml	Milli-Q water	filtration	2.5 ml
Carbenicillin, Disodium Salt	250 mg/ml	Milli-Q water	filtration	500 µl