Subject Area	Agricultural and Biological Sciences
More specific subject area	Transient gene expression in maize endosperm
Method name	Transient expression by biolistic bombardment in developing maize endosperm
Name and reference of original method	Plant material, In vitro endosperm culture, and aleurone bombardment pressure:Gruis, D. F., Guo, H., Selinger, D., Tian, Q., & Olsen, O. A. (2006). Surface position, not signaling from surrounding maternal tissues, specifies aleurone epidermal cell fate in maize. Plant Physiology, 141(3), 898–909.Gold particle preparations and bombardment chamber setup:Wang, K., & Frame, B. (2009). Biolistic gun-mediated maize genetic transformation. In Transgenic maize (pp. 29–45). Humana Press, Totowa, NJ.Whitham, S. A., Lincoln, L. M., Chowda‐Reddy, R. V., Dittman, J. D., O'Rourke, J. A., & Graham, M. A. (2016). Virus‐induced gene silencing and transient gene expression in soybean (Glycine max) using Bean pod mottle virus infectious clones. Current protocols in plant biology, 1(2), 263–283.
Resource availability	N.A.