KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-α-tubulin antibody (DM1A) | Thermo Fisher Scientific | Cat# 62204; RRID: AB_1965960 |
| anti-mouse IgG antibody | Sigma-Aldrich | Cat# F0257; RRID: AB_259378 |
| anti-rhodamine antibody | Thermo Fisher Scientific | Cat# A-6397; RRID: AB_2536196 |
| HRP-conjugated anti-rabbit antibody | Santa Cruz Biotechnology | Cat# sc-2004; RRID: AB_631746 |
| Bacterial and virus strains | ||
| Rosetta (DE3) pLysS E. coli | Novagen | -- |
| Biological samples | ||
| Adult rat ventricular myocytes | Purified from rats per methods | -- |
| Chemicals, peptides, and recombinant proteins | ||
| Dihydroethidium (DHE) | Sigma-Aldrich | Cat# D7008 |
| Amplex Red | ThermoFisher Scientific | Cat# A12222 |
| Experimental models: Cell lines | ||
| H9c2 cell line | ECACC | Cat# 88092904; RRID: CVCL_0286 |
| Recombinant DNA | ||
| CellLight Tubulin-GFP, BacMam 2.0 | Thermo Fisher Scientific | Cat# C10613 |
| Plasmid: pETMM11-HIS6x-Mal3-GFP | Gift from T. Surrey | MGP131 |
| Plasmid: HIS6x-TEVsite-MAL3-MCHERRY | Gift from T. Surrey | MGP106 |
| Software and algorithms | ||
| MATLAB | Mathworks | RRID:SCR_001622 |
| ImageJ - Fiji | NIH | RRID:SCR_002285 |