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. 2021 Mar 24;162(9):2405–2417. doi: 10.1097/j.pain.0000000000002226

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Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Description of experimental setup. (A) Mice were injected with AAV9 expressing GCaMP at least 5 days before imaging. (B) Mice were imaged with a one-photon microscope and (C) labelled cells visualised in the dorsal horn of the spinal cord. Scale bar = 100 μm. (D) Cartoon of cellular labelling after parabrachial injections. During imaging sessions the ipsilateral peripheral paw of the mouse was stimulated electrically (with a cuff electrode around the sciatic nerve), mechanically (through brush or pinch), or (E) thermally (using a Peltier device applied to the plantar surface of the paw). AAV, adeno-associated virus.