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. 2021 Aug 5;9:658112. doi: 10.3389/fcell.2021.658112

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Copyright © 2021 Sun, Tian, Xue, Ye, Xue, Wang, Liu, Zhang, Chen and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of REV-ERBs inhibits autophagy in KGN cells. KGN cells transfected with empty vector, REV-ERBα, REV-ERBβ, shRNA control, shREV-ERBα, or shREV-ERBβ plasmids for 48 h. (A) The expression of LC3 and p62 in KGN cells was detected by western blotting. (B) The expression of LC3B in KGN cells was detected by immunofluorescence. Scale bars: 50 μm. Blue fluorescence represents DAPI staining; Red fluorescence represents LC3B expression. (C) Morphological observation of autophagy in KGN cells under transmission electron microscopy. Arrows indicate autophagosomes or autolysosomes. Scale bars: 1.0 μm. **P < 0.01, ***P < 0.001 vs. empty vector.