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. 2021 Aug 17;218(10):e20210250. doi: 10.1084/jem.20210250

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© 2021 Beilinson et al.

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Figure 7. — J_β gene segment usage in VDJ_β rearrangements. (A) Schematic of murine Tcrb with the two RCs containing D_β1 and J_β1 or D_β2 and J_β2 gene segments highlighted. Figure not to scale. (B) Tcrb ImmunoSEQ data from mice 5–7 wk of age in CD4⁺CD8⁺CD3ε^lo thymic T cells were analyzed for frequency of J_β1 and J_β2 gene cluster usage in VDJ recombination events using the D_β1 gene segment. Number of recombination events analyzed can be found in Table S8. Data are presented as mean with error bars indicating SEM. Statistical significance determined by two-tailed unpaired t tests (ns, P > 0.05; *, P ≤ 0.05; ****, P ≤ 0.0001).