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. 2021 Aug 17;218(10):e20210250. doi: 10.1084/jem.20210250

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© 2021 Beilinson et al.

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Figure S2. — Flow cytometry analyses of B and T cell development in WT and R1.PG mice and characterization of germline transcripts in developing lymphocytes in WT and R1.D708A mice. (A–C) Representative flow cytometry data of WT and R1.PG mice showing the populations from thymic T cells (A), splenic T cells (B), and bone marrow B cells (C). (D–G) Quantification of RNA transcripts (D, F, and G) or DNA accessibility (E) of ICAM2 or Jλ1 (D and E), DQ52 (F), or D_β1 and D_β2 (G) conducted on samples from three or four independent samples of sorted preB cells (D and E), CD19⁺ cells from total bone marrow (F), or total thymocytes (G) from RAG1^−/− and R1.D708A (DA) mice with (D and E) or without (F) an IgH transgene. Signals from each assay were normalized to values from assay of the HPRT (D), CD19 (F), or Lck (G) gene. Shown are mean values ± SEM. Statistical significance determined by two-tailed unpaired t tests (ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001). FSC-A, forward scatter-A.