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. 2021 Aug 17;218(10):e20210250. doi: 10.1084/jem.20210250

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© 2021 Beilinson et al.

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Figure S3. — D_H gene segment usage and analysis of productive and nonproductive rearrangements in VDJ_H rearrangements in spleen assessed by HTGTS–Rep-seq. (A–C) Igh HTGTS–Rep-seq data from mice 5–7 wk of age were analyzed for frequency of DFL16.1 (blue), DQ52 (salmon), and intervening 11 D_H gene segments (green) in total (A) or nonproductive (B) VDJ_H or DJ recombination events (C) with J_H1 or J_H3 in spleen. Percentage of DQ52 usage is indicated above each bar. n, number of mice analyzed per genotype. (D) Frequency of productive (VDJ⁺) and nonproductive (VDJ⁻) recombination events at Igh in spleen with J_H1 or J_H3 using HTGTS–Rep-seq data. Number of recombination events analyzed can be found in Table S1, Table S2, and Table S3. Data are presented as mean with error bars indicating SEM. Statistical significance determined by two-tailed unpaired t tests (ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001).