Table 3. Evaluation of Intraday and Interday Accuracy and Precision of the Proposed Method in Case of Different Serum Samples.
| standard method3 | proposed
method |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
intraday
precision |
interday
precision |
|||||||||||||||
| serum sample | average ± RSD% | readings | average | RSD% | recovery% | t-test | F-test | average reading | CL | RE%a | RSD% | average reading | CL | RE% | RSD% | ||
| 1 | 12.1 ± 1.97 | 12.2 | 12.7 | 11.5 | 12.1 | 2.07 | 100.83 | 0.08 | 0.89 | 12.2 | 0.63 | 0.83 | 2.07 | 12.4 | 0.66 | 2.5 | 2.17 |
| 2 | 26.0 ± 1.72 | 25.5 | 26.7 | 27.2 | 26.4 | 1.42 | 101.53 | 0.46 | 1.42 | 26.7 | 0.95 | 1.53 | 1.42 | 26.8 | 1.02 | 3.07 | 1.52 |
| 3 | 29.1 ± 1.51 | 29.6 | 28.5 | 30.6 | 29.5 | 1.61 | 101.72 | 0.59 | 0.85 | 29.8 | 1.20 | 1.72 | 1.61 | 30.4 | 1.05 | 3.17 | 1.41 |
| 4 | 19.0 ± 1.42 | 17.6 | 18.9 | 19.0 | 18.5 | 1.92 | 97.36 | 1.25 | 0.57 | 18.9 | 0.90 | 2.63 | 1.92 | 19.4 | 0.93 | 2.63 | 1.98 |
| 5 | 18.0 ± 2.1 | 19.0 | 20.0 | 18.0 | 19.0 | 3.6 | 105.55 | 1.63 | 0.30 | 19.3 | 1.74 | 5.55 | 3.60 | 19.6 | 1.69 | 6.66 | 3.5 |
| 6 | 77.0 ± 1.23 | 76.9 | 77.9 | 77.6 | 77.4 | 1.39 | 100.51 | 0.07 | 0.77 | 77.6 | 2.73 | 0.51 | 1.39 | 80.6 | 2.93 | 4.41 | 1.49 |
| 7 | 98.0 ± 1.87 | 97.8 | 98.6 | 96.0 | 97.4 | 1.83 | 99.38 | 0.05 | 1.05 | 97.8 | 4.53 | 0.61 | 1.83 | 99.6 | 4.93 | 1.42 | 1.99 |
| 8 | 120 ± 0.89 | 122.7 | 121.0 | 121.6 | 121.7 | 1.02 | 101.41 | 1.07 | 0.74 | 121.9 | 3.15 | 1.41 | 1.02 | 124.2 | 4.08 | 3.33 | 1.32 |
| 9 | 84.0 ± 1.43 | 83.8 | 84.5 | 82.8 | 83.7 | 1.5 | 99.64 | 0.02 | 0.91 | 83.6 | 3.19 | 0.35 | 1.50 | 85.4 | 2.76 | 1.90 | 1.3 |
| 10 | 109.0 ± 0.88 | 108.8 | 110.6 | 109.6 | 109.6 | 1.01 | 100.55 | 0.16 | 0.75 | 109.8 | 2.81 | 0.55 | 1.01 | 112.2 | 5.60 | 2.75 | 2.01 |
| 11 | 113.0 ± 1.97 | 112.0 | 114.0 | 115.0 | 113.7 | 2.1 | 100.61 | 0.04 | 0.86 | 113.5 | 6.07 | 0.61 | 2.10 | 116.2 | 6.36 | 2.65 | 2.2 |
| 12 | 104.0 ± 2.71 | 106.0 | 107.0 | 103.0 | 104.7 | 2.98 | 100.67 | 0.02 | 0.81 | 104.9 | 7.94 | 0.67 | 2.98 | 106.2 | 8.26 | 1.90 | 3.1 |
| 13 | 198.0 ± 2.49 | 200.0 | 196.0 | 201.0 | 199.0 | 2.65 | 100.50 | 0.02 | 0.87 | 199.4 | 13.42 | 0.50 | 2.65 | 201.2 | 14.94 | 1.51 | 2.95 |
| 14 | 189.0 ± 2.87 | 192.0 | 187.0 | 191.0 | 190.0 | 3.1 | 100.52 | 0.02 | 0.84 | 190.6 | 14.99 | 0.52 | 3.10 | 192.2 | 13.10 | 1.58 | 2.71 |
| 15 | 230.0 ± 1.91 | 231.0 | 234.0 | 237.0 | 234.0 | 2.1 | 101.73 | 0.36 | 0.79 | 234.2 | 12.50 | 1.73 | 2.10 | 237.2 | 11.31 | 3.04 | 1.9 |
a
RE: percent relative error, %RSD: relative standard deviation, and CL: confidence limits were calculated from: CL = ±tS/√n. (the tabulated value of t is 4.303 at the 95% confidence level; S = standard deviation, and n = number of measurements). Theoretical values of t- and F-tests at 95% confidence limits are 4.303 and 19.0, respectively.