TABLE 1.

Comparative analysis of flagellar beating and kinematics across sperm from wild type and Crisp knock out mice.

(A) The calculated Procrustes measure of flagellar beat across individual wild type mice lines. Wild type vs. Knock out
								Procrustes measure ± SD				P value
Crisp1+/+ vs. Crisp1–/–								7.9 ± 1.8				<0.0001
Crisp2+/+ vs. Crisp2–/–								4.5 ± 1.4				<0.001
Crisp4+/+ vs. Crisp4–/–								7.6 ± 1.5				<0.0001
Crisp1/4+/+ vs. Crisp1/4–/–								2.5 ± 1.1				<0.001
(B) Analysis of power dissipation (in 10–8 J/s ±SD) at different regions of flagella in the presence or absence of Crisp genes
Region on the flagella	Crisp1		P Value	Crisp2		P Value	Crisp4		P Value	Crisp1/4		P Value
	WT	KO		WT	KO		WT	KO		WT	KO
Mid-piece	1.5 ± 0.29	0.5 ± 0.15	<0.0001	1.5 ± 0.29	0.5 ± 0.3	<0.0001	1.6 ± 0.4	0.24 ± 0.09	<0.0001	1.5 ± 0.24	1.16 ± 0.39	<0.0001
Principal piece	4.03 ± 0.72	1.5 ± 0.34	<0.0001	4.4 ± 0.86	2.4 ± 0.8	<0.0001	4.1 ± 0.89	1.57 ± 0.35	<0.0001	4.1 ± 0.96	2.07 ± 0.46	<0.0001
End piece	5.4 ± 0.83	2.5 ± 0.6	<0.0001	5.3 ± 1.2	3.4 ± 0.72	<0.0001	5.1 ± 0.8	2.9 ± 0.76	<0.0001	5.2 ± 0.9	2.5 ± 0.69	<0.0001

Please refer to Supplementary Table 4 for all the possible interactions between wild type vs. knock out genotype.