Fig. 4.

Gastrin induces angiogenesis after myocardial infarction. a1 Immunofluorescence staining with anti-cluster of differentiation 31 (CD31) antibody showed the microvessel densities in the infarcted border zone 14 days after MI. DAPI is blue, and CD31 is red. Scale bar = 50 µm. a2 Western blot was used to measure the protein levels of CD31 in the infarcted border zone of the heart 14 days after MI. Results are expressed as the ratio of CD31 to GAPDH. n = 8. ^*P < 0.05, vs. MI mice. b Human coronary artery endothelial cells (HCAECs) were incubated with gastrin at the indicated concentrations. Representative images (b1) and quantification (b2) of tube formation assay with HCAECs. Scale bar = 50 µm. n = 4. ^*P < 0.05, vs. Control