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. 2021 Aug 19;21:436. doi: 10.1186/s12935-021-02143-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Inhibition of BCL10 downregulates cell cycle proteins expression and NF-κB activation in PDAC cell lines. a In total cell lysates, transfection with shBCL10 downregulated the expression of p-CDC2, p-CDC25C, Cyclin B1 in PANC-1 cell lines and the expression of Cyclin A, E, and D1, CDK2, and CDK4 in BxPC-3 compared to scrambled (Scr) group and control (Con) group. When compared to scrambled (Scr) group and control (Con) group, shBCL10 transfection upregulated the expression of p21 and p27 in both PANC-1 and BxPC-3 cells. b shBCL10 transfection decreased the expressions of BCL3, c-Myc, p-IκBα, and NF-κB (p65) compared to scrambled (Scr) group and control (Con) group in nuclear lysates of both PANC-1 and BxPC-3 cells. c The results from three independent experiments using shBCL10-transfected PANC-1 and BxPC-3 cells are presented as relative luciferase units (RLU) per milligram of protein (NF-κB-Luc promoter activity-luciferase assay) (**p < 0.01). d PANC-1 cells and BxPC-3 cells were treated with BAY117082 (2 µM) for 24 h and compared with vehicle (0.01% DMSO) treated groups. *p < 0.05; n = 3. e PANC-1 cells and BxPC-3 cells were treated as described in (d), the expressions of BCL10, BCL3, p-p65, and p65 in nuclear lysates of PANC-1 cells and BxPC-3 cells, and the expressions of Cyclin B1, Cyclin D1, and p21 in total lysates were determined by western blotting. β-actin was used as the loading control. PDAC pancreatic ductal adenocarcinoma