Fig. 5.

NLRP3 was identified as the target of METTL14. A MeRIP-qPCR analysis of NLRP3 5'-UTR and 3'-UTR m⁶A levels in D-HNPC transduced with METTL3 shRNA or expression vector. B Relative expression of NLRP3 in D-HNPC transduced with METTL14 shRNA or expression vector was detected by qRT-PCR or western blot. C Luciferase activity was measured in D-HNPC transduced with METTL14 shRNA or expression vector. D The NLRP3 mRNA level was quantified by qRT-PCR in D-HNPC transfected with IGF2BP1, IGF2BP2, or IGF2BP3 siRNA. E The NLRP3 transcript was measured by qRT-PCR in D-HNPC transfected with IGF2BP2 siRNA. F The binding of IGF2BP2 to NLRP3 mRNA was measured by RIP and qRT-PCR