Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 18;19:163. doi: 10.1186/s12915-021-01084-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Structure and transcriptional control of five phase-variable capsular polysaccharide (CPS) operons (PVR7, PVR8, PVR9, PVR11, PVR12) in B. intestinalis APC919/174. a Host transcriptional response in a B. intestinalis APC919/174-ΦcrAss001 serial broth co-culture (24-120h in vitro persistence) experiment, analyzed using RNAseq (values are log₂ fold gene transcript expression change relative to the un-infected control from two independent experimental runs); clone-8T, phage-resistant derivative of the parental strain; clone-8W, spontaneous phage-sensitive revertant clone; only genes with significantly changed expression (p < 0.01 in DESeq2, n = 826) are shown; genes, associated with phase-variable genomic regions (PVR) are marked with color bars on top; b structure of phase variable operons, encoding five different CPS; protein sequence homologies (tBLASTx) between gene products are shown as colored parallelograms; two-sided arrows and ON/OFF labels mark positions of invertible promoters; c consensus sequence of the regions flanking invertible promoters (ON orientation) in CPS biosynthesis loci; approximate transcription start was identified though analysis of RNAseq data; stacked area charts show RNAseq read coverage of regions adjacent to the promoter in four CPS loci; d, e fraction of concordantly-aligned Illumina read pairs supporting orientation of invertible promoters in ON direction; WT, wild type strain; clones 8T, PhR1-9, phage-resistant derivatives; 8W, phage-sensitive revertant; P18d/P23d, samples in e were taken from the long-term in vitro persistence experiment (also see Additional file 1: Fig. S3 for similar data on the long term in vivo persistence)