Figure 4.

Sp1 regulated the expression of Gm26669 and mediated the expression of ECM-related genes by acting on the Gm26669 promoter. (A-B) JASPAR identified five binding sites for Sp1 in the Gm26669 promoter region. (C) CHIP experiments were performed to investigate the changes in binding between Sp1 and the Gm26669 promoter in BUMPT cells when stimulated by TGF-β1. (D) qRT-PCR analysis of Gm26669 expression levels in TGF-β1-stimulated BUMPT cells following Sp1 siRNA transfection. (E-G) qRT-PCR, western blotting and densitometric analyses of CoL-I and Fn expression levels in TGF-β1-stimulated BUMPT cells following transfection with Sp1 siRNA and LV-Gm26669. *P<0.05, ^#P<0.05, ^&P<0.05, ^{^}P<0.05; data are presented as the mean ±SEM (n=3).