The effects of NCTD on the deposition of ECM in the kidneys of UUO mice following the injection of LV-Gm26669. (A) qRT-PCR analysis of the expression levels of Gm26669 in the kidneys of UUO mice following NCTD treatment. (B) GFP immunofluorescence in the kidneys of UUO mice injected with LV-Gm26669 and treated with NCTD (400×). (C) qRT-PCR analyses of Gm26669 expression levels in the kidneys of UUO mice injected with LV-Gm26669 and treated with NCTD. (D-F) qRT-PCR, western blotting and densitometric analyses of the expression levels of CoL-I and Fn in the kidneys of UUO mice injected with LV-Gm26669 and treated with NCTD. *P<0.05, #P<0.05, &P<0.05, ^P<0.05, data are presented as the mean ±SEM (n=4).