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NCTD inhibited the nuclear translocation of Sp1 in the kidneys of UUO mice. (A) HE staining of mouse kidneys and the IHC staining of CoL-1, Fn, and Sp1 in the kidneys of UUO mice following NCTD treatment (400×). (B) IF analysis of Sp1 in the kidneys of UUO mice following NCTD treatment (400×). (C-D) Western blotting and densitometric analyses of Sp1 expression in the kidneys of UUO mice following NCTD treatment. *P<0.05, #P<0.05; data are presented as the mean ± SEM (n=4).
