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NCTD inhibited the nuclear translocation of Sp1 in BUMPT cells when stimulated by TGF-β1. (A) IF analysis of Sp1 in BUMPT cells following TGF-β1 stimulation and NCTD treatment (400×). (B-G) Western blotting and densitometric analyses of Sp1 expression levels in whole cells, nuclei, and the cytoplasm following TGF-β1 stimulation and NCTD treatment. *P<0.05, #P<0.05; data are presented as the mean ± SEM (n=3).
