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. 2021 Jul 13;17(12):3000–3012. doi: 10.7150/ijbs.60866

Figure 6.

Figure 6

Pf4 acts downstream of Sec23a to inhibit MAPK/ERK activation. (A) KEGG pathway enrichment analysis for Pf4-related genes in GSE22301 and GSE46517 and prioritized MAPK/ERK pathway; (B) (left)Pf4 knockdown augmented ERK/MAPK activation in OL and POL-Sec23a-OE cells; (right) Quantitative analysis of the expressions of p-ERK in OL-N.C. ,OL-shPf4,POL-Sec23a-shNC and POL-Sec23a-shPf4 (C) (top) recombinant PF4 protein (450 ng/ml) treatment inhibited ERK/MAPK activation in OL-shSec23a and POL cell; (bottom) Quantitative analysis of the expressions of p-ERK in PBS and rPF4. (D) (left) PD98059 (20uM) treatment prevented the increase of p-ERK caused by Pf4 knockdown in OL-shPf4; (right) Quantitative analysis of the expressions of p-ERK in DMSO and PD98059. (E) Pf4 knockdown had no significant effect on AKT activation in OL cells; (F-I) PD98059 (20uM) treatment prevented the increase of OL cell invasion and migration caused by Pf4 knockdown (OL-shPf4 cells); (J,K) PD98059(20uM) treatment prevented the increase of colony formation in POL-Sec23a and OL cells caused by Pf4 knockdown (OL-shPf4 and POL-Sec23a-OE-shPf4 cells).(*p<.05, **p<.01,***p<.001).