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Trametinib combined with rapamycin induces cell death in NSCLC cells. (A) A549, PC9 and H1650 cells were treated with 50 nM rapamycin alone, or 50 nM trametinib alone, or in combination for 48 hours, and cells were stained by crystal violet. (B-D) A549, PC9 and H1650 cells were treated with 50 nM rapamycin alone, or 50 nM trametinib alone, or in combination for 24 hours, and the dead cells were detected by SYTOX Green staining.
