Table 1.
Sequences of primers and probe used for PCR and cDNA synthesis.
| Oligonucleotides | Identity | Sequence (5’-3’) |
|---|---|---|
| Primers | N2830 (OFP) | CTGCTTAAGCCTCAATAAAGCTTGCCT |
| N2831 (ORP) | CCTGTGCCTCTTCAGCTACCACCGATTGAG | |
| N2832 (IFP) | GACTCTGGTAACTAGAGATCCCTCAGA | |
| N2833 (IRP) | GAATCGAAGAAGAAGGTGGAGAGCAAGACA | |
| Probe | N2842 | (FAM)-CTCTCGACGCAGGACTCGGCTTGCTGA-(BHQ-1) |
| RT primers | N4101 | TTTTTTTTTTTTTTCAGAGCACTC |
| N4102 | TTTTTTTTTTTTTCAGAGCACTCAAG | |
| N4103 | TTTTTTTTTTTTTTCAGAGCACTCAAGG | |
| Genomic DNA primers/Probe | N2208 | GATCTGAGCCTGGGAGCTCTCTG |
| N2209 | GACTCTGGTAACTAGAGATCCCTCAGA | |
| N2210 | (FAM)-CTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCT-(TAMRA) |
OFP, Outer forward primer; ORP, Outer reverse primer; IFP, Inner forward primer; IRP, Inner reverse primer. Reverse primer sequences are presented as reverse complement.