Table 2.
The expected size of various HIV-1 early viral transcripts in the TILDA.
| mRNA | Transcript | Exon combination | The expected size of the PCR fragments (bp) |
|---|---|---|---|
| Tat | Tat 1 | 1.4.7 | (167 + 268+69) 504 |
| Tat 2 | 1.[2].4.7 | (167 + 50+268+69) 554 | |
| Tat 3 | 1.[3].4.7 | (167 + 74+268+69) 578 | |
| Tat 4 | 1.[2].[3].4.7 | (167 + 50+74+268+69) 628 | |
| Nef | Nef 1 | 1.7 | (167 + 69) 236 |
| Nef 2 | 1.5.7 | (167 + 69+69) 305 | |
| Nef 3 | 1.[2].5.7 | (167 + 50+69+69) 355 | |
| Nef 4 | 1.[3].5.7 | (167 + 74+69+69) 379 | |
| Nef 5 | 1.[2].[3].5.7 | (167 + 50+74+69+69) 429 | |
| Rev | Rev 1 | 1.4B.7 | (167 + 85+69) 321 |
| Rev 2 | 1.4A.7 | (167 + 91+69) 327 | |
| Rev 3 | 1.4C.7 | (167 + 109+69) 345 | |
| Rev 4 | 1.[2].4B.7 | (167 + 50+85+69) 371 | |
| Rev 5 | 1.[2].4A.7 | (167 + 50+91+69) 377 | |
| Rev 6 | 1.[2].4C.7 | (167 + 50+109+69) 395 | |
| Rev 7 | 1.[3].4B.7 | (167 + 74+85+69) 395 | |
| Rev 8 | 1.[3].4A.7 | (167 + 74+91+69) 401 | |
| Rev 9 | 1.[3].4C.7 | (167 + 74+109+69) 419 | |
| Rev 10 | 1.[2].[3].4B.7 | (167 + 50+74+85+69) 445 | |
| Rev 11 | 1.[2].[3].4A.7 | (167 + 50+74+91+69) 451 | |
| Rev 12 | 1.[2].[3].4C.7 | (167 + 50+74+109+69) 469 |
The exons in square brackets are the alternatively-spliced transcripts.