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Primer sequence design of TLR4, NF-κB, MyD88, TLR2, and TRAF6.
| Gene | Primer sequence (5′–3′) | |
| TLR4 | F:GAAAGTATGGTAGGGG TGAAAGCG | R:GTGAAGGCAGAGCCG AAAGG |
| NF-κB | F:GAAGGACAAGACCAAA TTCTCAGT | R:GCAGGCTATTGCTCAACACG |
| TRAF6 | F:ACTATTCAGCAGTT AGAGGGTCG | R:CCATTTTTGCAGTCAGCTCC |
| MyD88 | F:CTACTGCCCCAGCGACAT | R:CTACTGCCCCAGCGACAT |
| TLR2 | F:GGGCTGTCTGTCACCGAAC | R:GGATGTGCAACCTCCGTATT |
| ACTIN | F:AAGTGCGACGTGGACATCCG | R:GGGCGGTGATCTCCTTCTGC |
