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. 2020 Mar 17;45:163–176. doi: 10.3767/persoonia.2020.45.06

Table 3.

Primers for amplification of mating type gene fragments.

Target gene Primer name Primer sequence (5’ to 3’) Tm (°C) Fragment size (bp) Target area
MAT1-1-1 Cal_MAT111_F ATGCTTCCTCAGTCTTTGCT 53 330 graphic file with name per-2020-45-6-i001.jpg
Cal_MAT111_R CTTGAAYRGGGTTGGTGG
MAT1-1-3 Cal_MAT113_F CCTCCAGAAGTACCGACT 48 430 graphic file with name per-2020-45-6-i002.jpg
Cal_MAT113_R GCTGTCGTTCTTCTTCCT
MAT1-2-1 Cal_MAT121_F GCAAGGAYCGCCACCRAAT 58 240 graphic file with name per-2020-45-6-i003.jpg
Cal_MAT121_R GACACCTCKGCGTTTCTTCTCAG
MAT1-2-12 Cal_MAT1212_F TCATCAGTTTCGCCCATT 48 670 graphic file with name per-2020-45-6-i004.jpg
Cal_MAT1212_R CGTCGTACTTCTTCTTCCG