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. 2021 Aug 5;12:709019. doi: 10.3389/fphar.2021.709019

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Copyright © 2021 Wang, Yang, Yang, Ren, Feng, Liu, Chen and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rg1 decreases ubiquitination of Cx43 in rats exposed to CUS. The tissue lysates immunopreciptated using normal rabbit IgG or rabbit antibody against total Cx43 (IP: Cx43). The immune complexes immunoblotted using an antibody against ubiquitin (IB: Ubiquitin). The blots from three independent experiments were subjected to densitometric analyses for ubiquitinated Cx43 and the results were expressed as the density of the bands in the test sample relative to those in the vehicle sample. n = 3 per group. **p < 0.01, compared with the vehicle group; ^# p < 0.05, compared with the CUS group. Cx43, connexin 43.