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. 2021 Aug 5;12:709019. doi: 10.3389/fphar.2021.709019

TABLE 1.

Chronic unpredictable stress procedure.

Day Stressors
Monday Isolation 24 h (one rat per cage); cage tilt 45°overnight
Tuesday Tail pinch 3 min; Crowding overnight (15 rats per cage)
Wednesday Electric shock 3 min (0.8 mA); water deprivation 12 h
Thursday White noise 1 h (110 dB); strobe flash 2 h
Friday Swim stress 10°C, 5 min; shaker stress 1 h (160 rpm)
Saturday Restraint stress 4 h; reversal of the light/dark cycle (light off 12 h and light on overnight)
Sunday Food deprivation 12 h; wet bedding overnight