Figure 5.

Effects of PCOS fecal microbiota transplantation on the disruption of insulin sensitivity. Mice transplanted with stool suspensions from healthy controls and women with PCOS were defined as the control-FMT and PCOS-FMT groups, respectively. After treatment with an antibiotic cocktail (20 mg/mL vancomycin, 40 mg/mL neomycin sulfate, 40 mg/mL metronidazole, 40 mg/mL ampicillin intragastrically once daily) for 5 days, the mice were orally gavaged with stool suspensions once a day for 1 week and once every 3 days for 9 weeks (control-FMT: 8; PCOS-FMT: 8). (A) Schematic representation of the experimental design. (B) Serum testosterone levels. (C) GTT. (D) Fasting blood glucose. (E) Fasting insulin. (F) HOMA-IR. (B-E) P values were determined by 2-tailed Student t test, and P < 0.05 was considered statistically significant. FMT, fecal microbiota transplantation; GTT, glucose tolerance test; HOMA-IR, homeostasis model assessment for insulin resistance index; PCOS, polycystic ovarian syndrome.