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. Author manuscript; available in PMC: 2021 Aug 19.
Published in final edited form as: Mol Pharm. 2012 Oct 22;9(11):3147–3159. doi: 10.1021/mp300249s

Figure 3.

Figure 3.

BBA was not a transported substrate of ABCB1 and had persistent reversal activity. (A) Cytotoxicity of BBA on drug-sensitive HepG2 and drug-resistant HepG2/ADM cells. (B) Effect of verapamil and cyclosporine A on the sensitivity of BBA in ABCB1-overexpressing HepG2/ADM cells. Cells were treated with various concentrations of BBA in the presence or absence of verapamil (5 μM) or cyclosporine A (5 μM) for 48 h, and cytotoxicity of BBA was detected by MTT assay. The mean values are plotted, and the error bars depict standard of deviation from at least three independent experiments. (C) Duration of BBA reversing DOX resistance in HepG2/ADM cells. Cells were treated with or without BBA (1.25 and 5 μM) or verapamil (5 μM) for 24 h before being washed out, then treated with DOX for 48 h. Cytotoxicity of DOX was detected by MTT assay. The mean values are plotted, and the error bars depict standard of deviation from at least three independent experiments.