Fig. 1.

HIF-1α mRNA, HIF-1α protein, and HIF-1α target gene mRNA levels were determined in paired tumor biopsies collected at baseline (pre-dose) and following the administration of EZN-2968. a HIF-1α and B2M mRNA copy numbers were determined in comparison with a plasmid DNA standard curve; HIF-1α mRNA copy number was then normalized to mRNA copy number for B2M. Percentage indicates overall change from baseline. b HIF-1α protein levels in 5 patients; percentage indicates overall change from baseline. *Pre-dose biopsy <LLQ; +post-dose biopsy <LLQ. c RT-PCR for HIF-1α target genes VEGF, CAIX, GLUT-1, and PDK-1. Results expressed as mRNA fold change in EZN-2968 treatment samples relative to baseline, arbitrarily considered equal to 1