Skip to main content
. 2021 Aug 5;12:629276. doi: 10.3389/fphys.2021.629276

FIGURE 1.

FIGURE 1

Sinomenine treatment attenuated capsaicin-induced cough sensitivity in guinea pigs. (A) Cough reflex sensitivity (CRS) in guinea pigs. Sinomenine inhibited the cough induced by the capsaicin. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. (B) Sinomenine reduced the capsaicin-induced increase in Penh. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. (C) H&E staining of lung tissues showing the levels of infiltration by inflammatory cells. Scale bars represent 50 and 100 μm. (D,F) CD68 staining of lung tissues from guinea pigs treated with saline, capsaicin, or sinomenine. Scale bars represent 50 and 100 μm. (E,G) Western blot assay of the expression levels of SP and NKA in trachea and lung tissues of guinea pigs. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. (H) Scheme for the screening of guinea pigs and construction of cough model. **P < 0.01; n = 7.