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. 2021 Aug 5;12:629276. doi: 10.3389/fphys.2021.629276

FIGURE 2.

FIGURE 2

Sinomenine inhibited the expression of TRPV1 induced by capsaicin in neuronal cell. (A) Expression level of TRPV1 in neuronal cells, as measured using western blot analysis. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. (B) qRT-PCR analysis showing the mRNA level of TRPV1 in neuronal cells. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. (C) Intracellular calcium levels. Sinomenine inhibited capsaicin-induced calcium uptake in neuronal cells. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. (D) Protein expression levels of SP and NKA were reduced by sinomenine and elevated after TRPV1 overexpression in neuronal cells. (E) CRS levels showing that effect of sinomenine was inhibited after TRPV1 administration. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. (F) Effect of TRPV1 administration on Penh. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. **P < 0.01; n = 7.