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. 2021 Aug 5;12:629276. doi: 10.3389/fphys.2021.629276

FIGURE 6.

FIGURE 6

Effect of sinomenine on capsaicin-induced increase in cough sensitivity in guinea pigs, and the implication of SOX5 and TRVP1 in the process. (A,B) Protein expression levels of TRPV1 and SOX5 in guinea pigs, as assayed using western blotting. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. (C) CRS in guinea pigs showing that treatment by sinomenine and SOX5 increased the number of cough. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. (D) SOX5 administration enhanced Penh. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. (E) H&E staining showing levels of inflammatory cell infiltration. Scale bars represent 50 and 100 μm. (F,I) CD68 staining indicating that SOX5 increased the infiltration of monocytes/macrophages. Scale bars represent 50 and 100 μm. (G,H) Western blot analysis showing the expression levels of SP and NKA in guinea pigs. Data are mean ± SEM; one-way ANOVA was used for statistical analysis. **P < 0.01; n = 7.