Skip to main content
. 2021 Aug 9;17(8):e1009729. doi: 10.1371/journal.pgen.1009729

Fig 1. Robust inductions of Dll1 expression by myogenic cues in both mouse and human cells.

Fig 1

(A) Human myoblasts display high myogenic and fusogenic potentials in culture. Cells were labelled by GFP to visualize the syncytium at early stages of differentiation. Scale bar, 100 μm. (B) Western blotting results of myosin (MF20 antibody) and Myogenin (MyoG) in human myoblasts at various stages of differentiation. (C) qPCR results of Notch ligand genes during human myoblast differentiation. The Ct values for each gene at growth-culture condition (time 0) are Dll1 (25.6), Dll3 (26.8), Dll4 (32.4) and Jag1 (28.6). Jag2 expression was not detected. (D, E) RNA sequencing results of Notch ligand genes and Notch target Heyl during mouse myoblast differentiation (D, GSE20846) and muscle regeneration (E, GSE97764). CTX (cardiotoxin) samples were from day 3 post injury. FC, expression fold change.