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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: J Pharmacol Sci. 2020 Dec 29;145(4):319–326. doi: 10.1016/j.jphs.2020.12.006

Fig. 5.

Fig. 5.

Effect of ABK5 on CXCL12 mediated Jurkat cell migration. (A) Jurkat cell migration in response to different concentrations of CXCL12. Various concentrations of CXCL12 were added to lower chamber and cells were allowed to migrate for 2 h. Cell numbers migrated to the lower chamber and are indicated as % cell migrated relative to cell numbers loaded on the top chamber. (B) Transwell migration of Jurkat cells. Cells were treated with vehicle or various concentrations of ABK5 for 2 h and then allowed to migrate through transwells for 2 h. Cell numbers migrated to the lower chamber are indicated as relative to vehicle (DMSO) alone. Results are indicated as the mean ± S.E.M. of three independent assays performed in triplicate for each concentration. (C) Cell viability after the migration assay. Cells were treated exactly the same but without transwells and viability was determined. Results are indicated as the mean ± S.E.M. of three independent assays performed for each concentration. One-way ANOVA plus Dunnett’s post-hoc test were used for both migration and viability assays. **p < 0.01, ***p < 0.001, ****p < 0.0001 versus vehicle control group.