| Gene (Mus musculus TMEM120A) |
M. musculus TACAN |
Synthetic |
|
Synthesized at GeneWiz. |
| Gene (Homo sapiens TMEM120A) |
H. sapiens TACAN |
Synthetic |
|
Synthesized at GeneWiz. |
| Gene (Homo sapiens ELOVL7) |
H. sapiens ELOVL7 |
Synthetic |
|
Synthesized at GeneWiz. |
| Strain, strain background (Escherichia coli) |
DH10Bac |
Thermo Fisher Scientific |
10361012 |
|
| Recombinant DNA reagent |
TACAN-eGFP BacMam |
This study |
|
|
| Recombinant DNA reagent |
ELOVL7-eGFP BacMam |
This study |
|
|
| Recombinant DNA reagent |
Halo-M2R-eGFP BacMam |
This study |
|
|
| Cell line (Spodoptera frugiperda) |
Sf9 |
ATCC |
CRL-1711 |
Cells purchased from ATCC, and we have confirmed there is no mycoplasma contamination |
| Cell line (Chinese hamster) |
CHO-K1 |
ATCC |
CRL-9618 |
Cells purchased from ATCC, and we have confirmed there is no mycoplasma contamination |
| Cell line (Homo sapiens) |
HEK293S GnTI-
|
ATCC |
CRL-3022 |
Cells purchased from ATCC, and we have confirmed there is no mycoplasma contamination |
| Cell line (Homo sapiens) |
Piezo1 knockout HEK293T |
https://digitalcommons.rockefeller.edu/cgi/viewcontent.cgi?article=1422&context=student_theses_and_dissertations
|
|
We have confirmed there is no mycoplasma contamination |
| Chemical compound, drug |
SF-900 II SFM medium |
Gibco |
11330-032 |
|
| Chemical compound, drug |
L-Glutamine (100×) |
Gibco |
25030-081 |
|
| Chemical compound, drug |
Pen Strep |
Gibco |
15140-122 |
|
| Chemical compound, drug |
Grace’s insect medium |
Gibco |
11605-094 |
|
| Chemical compound, drug |
Freestyle 293 medium |
Gibco |
12338-018 |
|
| Chemical compound, drug |
DMEM/F-12 medium |
Gibco |
11605-094 |
|
| Chemical compound, drug |
DMEM |
Gibco |
11965-118 |
|
| Chemical compound, drug |
Fetal bovine serum |
Gibco |
16000-044 |
|
| Chemical compound, drug |
Cellfectin II reagent |
Invitrogen |
10362100 |
|
| Chemical compound, drug |
FuGENE HD transfection reagent |
Promega |
E2312 |
|
| Chemical compound, drug |
Cholesteryl hemisuccinate (CHS) |
Anatrace |
CH210 |
|
| Chemical compound, drug |
n-Decyl-β-D-maltopyranoside (DM) |
Anatrace |
D322S |
|
| Chemical compound, drug |
Lauryl maltose neopentyl glycol (LMNG) |
Anatrace |
NG310 |
|
| Chemical compound, drug |
Digitonin |
Millipore Sigma |
300410 |
|
| Chemical compound, drug |
Coenzyme A trilithium salt (CoASH) |
Sigma-Aldrich |
C3019 |
|
| Chemical compound, drug |
Acetyl coenzyme A sodium salt (acetyl-CoA) |
Sigma-Aldrich |
A2056 |
|
| Chemical compound, drug |
S-Ethyl-coenzyme A sodium salt (S-ethyl-CoA) |
Jena-Biosciences |
NU-1168 |
|
| Chemical compound, drug |
Malonyl coenzyme A lithium salt (malonyl-CoA) |
Sigma-Aldrich |
M4263 |
|
| Chemical compound, drug |
Stearoyl coenzyme A lithium salt (stearoyl-CoA) |
Sigma-Aldrich |
S0802 |
|
| Chemical compound, drug |
(1H, 1H, 2H, 2H-Perfluorooctyl)phosphocholine (FFC8) |
Anatrace |
F300F |
|
| Commercial assay or kit |
CNBr-activated Sepharose beads |
GE Healthcare |
17-0430-01 |
|
| Commercial assay or kit |
Superdex 200 Increase 10/300 GL |
GE Healthcare Life Sciences |
28990944 |
|
| Commercial assay or kit |
R1.2/1.3 400 mesh Au holey carbon grids |
Quantifoil |
1210627 |
|
| Commercial assay or kit |
Coenzyme A (CoA) Assay Kit |
Sigma-Aldrich |
MAK034 |
|
| Software, algorithm |
RELION 3.0 |
https://doi.org/10.7554/eLife.42166.001
|
http://www2.mrc-lmb.cam.ac.uk/relion
|
|
| Software, algorithm |
RELION 3.1 |
https://doi.org/10.1101/798066
|
http://www2.mrc-lmb.cam.ac.uk/relion
|
|
| Software, algorithm |
MotionCor2 |
https://doi.org/10.1038/nmeth.4193
|
http://msg.ucsf.edu/em/software/motioncor2.html
|
|
| Software, algorithm |
Gctf 1.0.6 |
https://doi.org/10.1016/j.jsb.2015.11.003
|
https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/
|
|
| Software, algorithm |
CtfFind4.1.8 |
https://doi.org/10.1016/j.jsb.2015.08.008
|
http://grigoriefflab.janelia.org/ctffind4
|
|
| Software, algorithm |
CryoSPARC 2.9.0 |
https://doi.org/10.7554/eLife.46057.001
|
https://cryosparc.com/
|
|
| Software, algorithm |
COOT |
https://doi.org/10.1107/S0907444910007493
|
http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
|
|
| Software, algorithm |
PHENIX |
https://doi.org/10.1107/S0907444909052925
|
https://www.phenix-online.org
|
|
| Software, algorithm |
Adobe Photoshop version 16.0.0 (for figure preparation) |
Adobe Systems, Inc. |
|
|
| Software, algorithm |
GraphPad Prism version 8.0 |
GraphPad Software |
|
|
| Software, algorithm |
MacPyMOL: PyMOL v2.0 Enhanced for Mac OS X |
Schrodinger LLC |
https://pymol.org/edu/?q=educational/
|
|
| Software, algorithm |
Chimera |
https://doi.org/10.1002/jcc.20084
|
https://www.cgl.ucsf.edu/chimera/download.html
|
|
| Software, algorithm |
Serial EM |
https://doi.org/10.1016/j.jsb.2005.07.007
|
http://bio3d.colorado.edu/SerialEM
|
|
| Software, algorithm |
pClamp |
Axon Instruments, Inc |
|
|
| Software, algorithm |
Thermo Xcalibur Qual Browser (v. 4.2.47) |
Thermo Fisher Scientific |
|
|
| Software, algorithm |
UniDec v. 4.2.0 |
Marty et al., 2015; Reid et al., 2018
|
https://github.com/michaelmarty/UniDec/releases
|
|
