Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | TOP10 | Thermo Fisher Scientific | Cat# 18258012 | |
| Strain, strain background (E. coli) | DH10bac | Thermo Fisher Scientific | Cat# 10361012 | |
| Cell line (Spodoptera frugiperda) | Sf9 cells | Thermo Fisher Scientific | Cat# 11496015; RRID:CVCL_0549 | |
| Cell line (Homo sapiens) | FreeStyle 293 F cells | Thermo Fisher Scientific | Cat# R79007; RRID:CVCL_D603 | |
| Transfected construct (H. sapiens) | pEZT-BM-TMEM120A-Nflag | This paper | N/A | |
| Recombinant DNA reagent | pEZT-BM | DOI:10.1016/j.str.2016.03.004 | Addgene:74099 | |
| Sequence-based reagent | TMEM120A_F_primer: gatataGCTAGCCAACCGCCACCACCCGGGCCATTG | This paper | N/A | |
| Sequence-based reagent | TMEM120A_R_primer: gatataGCGGCCGCTCAATCTTTTTTTGAGCCATG | This paper | N/A | |
| Peptide, recombinant protein | Flag peptide | Sigma-Aldrich | Cat# F3290 | |
| Commercial assay or kit | Coenzyme A Assay Kit | Sigma-Aldrich | Cat# MAK034 | |
| Chemical compound, drug | Sodium Butyrate | Sigma-Aldrich | Cat# 303410 | |
| Chemical compound, drug | Lauryl Maltose Neopentyl Glycol | Anatrace | Cat# NG310 | |
| Chemical compound, drug | Digitonin | Acros Organics | Cat# 11024-24-1 | |
| Software, algorithm | MotionCor2 | Zheng et al., 2017 | http://msg.ucsf.edu/em/software/motioncor2.html | |
| Software, algorithm | GCTF | Zhang, 2016 | https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf | |
| Software, algorithm | RELION | Scheres, 2012 | http://www2.mrc-lmb.cam.ac.uk/relion | |
| Software, algorithm | Chimera | Pettersen et al., 2004 | https://www.cgl.ucsf.edu/chimera; RRID:SCR_004097 | |
| Software, algorithm | PyMol | Schrödinger | https://pymol.org/2; RRID:SCR_000305 | |
| Software, algorithm | COOT | Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot; RRID:SCR_014222 | |
| Software, algorithm | MolProbity | Chen et al., 2010 | http://molprobity.biochem.duke.edu/ | |
| Software, algorithm | PHENIX | Adams et al., 2010 | https://www.phenix-online.org | |
| Other | Superose 6 Increase10/300 GL | GE Healthcare | Cat# 29091596 | |
| Other | Anti-DYKDDDDK G1 Affinity Resin | GeneScript | Cat# L00432 | |
| Other | Amicon Ultra-15 Centrifugal Filter Units | Milliporesigma | Cat# UFC9100 | |
| Other | Quantifoil R 1.2/1.3 grid Au300 | Quantifoil | Cat# Q37572 | |
| Other | Cellfectin | Thermo Fisher Scientific | Cat# 10362100 | |
| Other | Sf-900 II SFM medium | Thermo Fisher Scientific | Cat# 10902088 | |
| Other | FreeStyle 293 Expression Medium | Thermo Fisher Scientific | Cat# 12338018 | |
| Other | Antibiotic Antimycotic Solution | Sigma-Aldrich | Cat# A5955 | |
| Other | Proteinase K | Thermo Fisher Scientific | Cat# EO0491 |