Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Rabbit polyclonal anti-GIV (Girdin) (T-13) | Santa Cruz Biotechnology | sc-133371 | |
| Antibody | Rabbit monoclonal diagnostic grade anti-Girdin/GIV antibody | Custom; Sprint Bioscience | SP173 | Validated in prior publication Ghosh et al., 2016 |
| Antibody | Rabbit polyclonal anti-GIV (Girdin) (CC-Ab) | Millipore Sigma | ABT80 | |
| Antibody | Rabbit polyclonal anti-GIV pS1675 Ab | Custom, from 21t Century Biosciences | n/a | Validated in prior publication Bhandari et al., 2015 |
| Antibody | Rabbit polyclonal anti-GIV pS1689 Ab | Custom, from 21st Century Biosciences | n/a | Validated in prior publication López-Sánchez et al., 2013 |
| Antibody | Rabbit monoclonal anti-GIV pY1764 Ab | Custom, Spring Biosciences Inc | n/a | Validated in prior publications Midde et al., 2015; Lin et al., 2011; Midde et al., 2018; Dunkel et al., 2016 |
| Antibody | Rabbit monoclonal anti-pT308 AKT | Cell Signaling Technology | D9E | |
| Antibody | Mouse monoclonal anti-total AKT | Cell Signaling Technology | 40D4 | |
| Antibody | Mouse anti-sp56 | Thermo Fisher Scientific (Waltham, MA) | MA1-10866 | |
| Antibody | Mouse anti- human hexokinase 1/2 monoclonal antibody | R&D Systems, (Minneapolis, MN) | MAB8179 | |
| Antibody | Rabbit anti-phospho-PKA substrate (RRXS*/T*)100G7E | Cell Signaling Technology | 9624 | |
| Antibody | Goat anti-rabbit IgG, Alexa Fluor 594 conjugated | ThermoFisher Scientific | A11072 | For immunofluorescence (IF) |
| Antibody | Goat anti-mouse IgG, Alexa Fluor 488 conjugated | ThermoFisher Scientific | A11017 | For immunofluorescence (IF) |
| Antibody | IRDye 800CW goat anti-mouse IgG secondary (1:10,000) | LI-COR Biosciences | 926-32210 | For immunoblotting |
| Antibody | IRDye 680RD goat anti-rabbit IgG secondary (1:10,000) | LI-COR Biosciences | 926-68071 | For immunoblotting |
| Strain, strain background (Mus musculus) | UbcCre-Ert2/+ xCcdc88afl/fl andCcdc88afl/fl mice | Masahide Takahashi (Nagoya University Graduate School of Medicine, Nagoya, Japan) | n/a | |
| Strain, strain background (Mus musculus) | WT and GIV-cKO (conditional KO) mice | This work | UbcCre-Ert2/+ xCcdc88afl/fl (experimental group), and UbcCre-Ert2/ Cre-Ert2 x Ccdc88afl/fl (control group) | This work; male |
| Strain, strain background (Mus musculus) | C57BL/6J mice (male and female) | The Jackson Laboratory | Stock number: 000664; Bar Harbor, ME | Male: source of sperm for biochemical, immunohistochemical, peptide transduction, and functional assaysFemale: for co-housing studies; source for eggs for IVF assays |
| Chemical compound | Paraformaldehyde 16% | Electron Microscopy Biosciences | 15710 | |
| Chemical compound | MTT | Millipore Sigma | 475989-1GM | |
| Other | DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) | Thermo Fisher Scientific | D3571 | Used in IF studies for staining DNA/nucleus |
| Kit/reagent | HisPurä Cobalt Resin | Thermo Scientific | 89964 | |
| Kit/reagent | Glutathione Sepharoseâ 4B | Sigma-Aldrich | GE17-0756-04 | |
| Chemical compound | Protease inhibitor cocktail | Roche | 11873580001 | |
| Chemical compound | Tyr phosphatase inhibitor cocktail | Sigma-Aldrich | P5726 | |
| Chemical compound | Ser/Thr phosphatase inhibitor cocktail | Sigma-Aldrich | P0044 | |
| Other | PVDF Transfer Membrane, 0.45 mM | Thermo Scientific | 88518 | Used for transfer in immunoblots |
| Commercial assay or kit | Countess II Automated Cell Counter | Thermo Fisher Scientific | AMQAX1000 | |
| Commercial assay or kit | Leica TCS SPE Confocal | Leica Microsystems | TCS SPE | |
| Commercial assay or kit | Light Microscope (brightfield images) | Carl Zeiss LLC | Axio Observer, Inverted; 491917-0001-000 | |
| Software | ImageJ | National Institute of Health | https://imagej.net/Welcome | |
| Software | Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ | |
| Software | LAS-X | Leica | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls | |
| Software | Illustrator | Adobe | https://www.adobe.com/products/illustrator.html | |
| Software | ImageStudio Lite | LI-COR | https://www.licor.com/bio/image-studio-lite/ |