Table 1. Sequencing statistics of longitudinal PBMC samples from subject QA013.

PBMC time point	Live PBMC count	PBMC viability	Ab chain	Raw MiSeq reads	Productive deduplicated sequences	Sequence coverage of sampled blood PBMCs	Sequence coverage of QA013 D2282 whole-body blood* repertoire
D-379	2.60E+06	96%	IgM	1358426	2700	0.8%	0.002%
			IgG	1357742	8350	14%	0.03%
			IgK	1701268	21043	6%	0.01%
			IgL	1528383	27458	7%	0.02%
D-351	2.50E+06	100%	IgM	1186865	2213	0.7%	0.002%
			IgG	1290351	7093	12%	0.03%
			IgK	1724651	19835	5%	0.01%
			IgL	1116583	23489	6%	0.01%
D385	3.90E+06	100%	IgM	1299494	2039	0.4%	0.001%
			IgG	1512588	6222	7%	0.02%
			IgK	2058137	22252	4%	0.01%
			IgL	1454466	27107	5%	0.01%
D765	6.80E+06	96%	IgM	1264049	2480	0.3%	0.001%
			IgG	1465034	7424	12%	0.03%
			IgK	2083938	21738	2%	0.00%
			IgL	1556226	26530	3%	0.01%

 *Assuming PBMC samples were from a 10 mL blood draw and that adults have 4500 mL total blood volume.