Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Naa10 | GenBank | MGI:MGI:1915255 | |
| Gene (M. musculus) | Naa15 | GenBank | MGI:MGI:1922088 | |
| Gene (M. musculus) | Naa11 | GenBank | MGI:MGI:2141314 | |
| Gene (M. musculus) | Naa12 | This paper | Gm16286, UniProt: Q9CQX6 | Provided by corresponding author, Gholson J. Lyon |
| Genetic reagent (M. musculus) | Naa10-/- | Nature Communication Yoon et al., 2014 | Provided by corresponding author, Goo Taeg Oh | |
| Genetic reagent (M. musculus) | Naa12-/- | This paper | Gm16286, UniProt: Q9CQX6 | Provided by corresponding author, Gholson J. Lyon |
| Cell line (Homo sapiens) | HEK293 (normal, embryonic kidney cells) | ATCC | CRL-1573 | |
| Biological sample (M. musculus) | Primary mouse embryonic fibroblasts | This paper | Freshly isolated from mouse embryos (E13.5) | |
| Antibody | Anti-Naa10 (rabbit polyclonal) | Abcam | Cat# ab155687 | (1:1000) |
| Antibody | Anti-Naa10 (rabbit polyclonal) | Protein Tech | Cat# 14803-1-AP | (1:3000) |
| Antibody | Anti-Naa10 (rabbit monoclonal) | Cell Signaling | Cat# 13357 | (1:1000) |
| Antibody | Anti-Naa10 (goat polyclonal) | Santa Cruz | Cat# sc-33256 | (1:1000) |
| Antibody | Anti-Naa10 (rabbit polyclonal) | Santa Cruz | Cat# sc-33820 | (1:1000) |
| Antibody | Anti-Naa11 (rabbit polyclonal) | Novus Biologicals | Cat# NBP1-90853 | (1:1000) |
| Antibody | Anti-Naa15/NARG1 (mouse monoclonal) | Abcam | Cat# ab60065 | (1:1000) |
| Antibody | Anti-NAA15 (rabbit polyclonal) | Biochemical Journal (reference 12 in this paper) Arnesen et al., 2005 |
(1:2000) Provided by author Thomas Arnesen, |
|
| Antibody | Anti-NAA50 (rabbit polyclonal) | LifeSpan BioSciences | Cat# LS-C81324-100 | (1:3000) |
| Antibody | Anti-FLAG (rabbit polyclonal) | Sigma-Aldrich | Cat# F7425 | (2 μg/mL) |
| Antibody | Anti-GAPDH (mouse monoclonal) | Abcam | Cat# ab9484 | (1:3000) |
| Antibody | Anti-actin (goat polyclonal) | Santa Cruz | Cat# 1615 | (1:3000) |
| Antibody | Anti-GST (mouse monoclonal) | GenScript | Cat# A00865 | (1 µg/mL) |
| Antibody | Anti-V5 (mouse monoclonal) | Life Technologies | Cat# R960-25 | (1:1000) |
| Antibody | Anti-Naa12 (rabbit polyclonal) | This paper | Gm16286, UniProt: Q9CQX6 | C-terminus (aa191-205: QENLAGGDSGSDGKD-C) conjugated to OVA by PrimmBiotech Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mNaa10-Exon2/3_F | This paper | PCR primers | ctcttggccccagctttctt Provided by corresponding author, Goo Taeg Oh |
| Sequence-based reagent | mNaa10-Exon3/4_R | This paper | PCR primers | tcgtctgggtcctcttccat Provided by corresponding author, Goo Taeg Oh |
| Sequence-based reagent | mNaa11_F | This paper | PCR primers | accccacaagcaaagacagtg Provided by corresponding author, Goo Taeg Oh |
| Sequence-based reagent | mNaa11_R | This paper | PCR primers | agcgatgctcaggaaatgctct Provided by corresponding author, Goo Taeg Oh |
| Sequence-based reagent | mNaa12(Gm16286)_F | This paper | PCR primers | acgcgtatgctatgaagcga Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mNaa12(Gm16286)__R | This paper | PCR primers | ccaggaagtgtgctaccctg Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mNaa15_F | This paper | PCR primers | gcagagcatggagaaaccct Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mNaa15_R | This paper | PCR primers | tctcaaacctctgcgaacca Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mNaa50_F | This paper | PCR primers | taggatgccttgcaccttacc Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mNaa50_R | This paper | PCR primers | gtcaatcgctgactcattgct Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mGAPDH_F | This paper | PCR primers | aggtcggtgtgaacggatttg Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mGAPDH_R | This paper | PCR primers | tgtagaccatgtagttgaggtca Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mACTB_F | This paper | PCR primers | ggctgtattcccctccatcg Provided by corresponding author, Gholson J. Lyon |
| Sequence-based reagent | mACTB_R | This paper | PCR primers | ccagttggtaacaatgccatgt Provided by corresponding author, Gholson J. Lyon |
| Software, algorithm | Zen 3.0 SR | ZEISS | Version 16.0.1.306 | Black 64bit edition |
| Other | Alcian Blue 8GX | Sigma-Aldrich | Cat# A5268 | 0.03% |
| Other | Alizarin Red | Sigma-Aldrich | Cat# A5533 | 0.05% |
| Other | Hematoxylin | Sigma-Aldrich | Cat# MHS80 | |
| Other | Eosin | Sigma-Aldrich | Cat# HT110116 |