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. 2021 Jul 28;10:e66192. doi: 10.7554/eLife.66192

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© 2021, Kwon et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — Using Monocle3, mosquito immune cells were visualized by UMAP to reveal two distinct lineages in pseudotime under naive (A), blood-fed (B), or combined (naïve and blood-fed) samples (C) with the corresponding immune cell clusters for each condition. Based on the lineage analysis, gene expression, and other functional assays, our data support the following model of immune cell development and differentiation where prohemocytes serve as precursors for the granulocyte and oenocytoid lineages (D). Each cell type is labeled with the corresponding cell cluster described in our analysis. Figure was created with BioRender.com.