Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (An. gambiae) | Keele |
Hurd et al., 2005; Ranford-Cartwright et al., 2016 |
NA | |
| Biological sample (An. gambiae) | adult female hemolymph | NA | NA | Perfused hemocytes from naïve (sugar-fed) or blood-fed (24 hr post-feeding) mosquitoes |
| Sequence-based reagent | LRIM15 (qRT-PCR primers) | Smith et al., 2016 | AGAP007045 |
F:CGATCCTGATCCTGAACGTGGGCTTC R:GCAAGCAAGCCACTCACAAATCCTCG |
| Sequence-based reagent | LRIM16A (qRT-PCR primers) | Smith et al., 2016 | AGAP028028 |
F:ATCAGAGTGCAGCACAAGTTGAAGGT R:TCTCTGTTAGCATAGCGCCTTCGTTC |
| Sequence-based reagent | Lz (qRT-PCR primers) | This study | AGAP002506 |
F:GCACCGTCAATCAGAACCAA R:TGCCACTGATCGAATGCTTG |
| Sequence-based reagent | NimB2 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP029054 |
F:CAATCTGCTCAAATGGCTGCTTCCACG R:GCTGCAAACATTCGGTCCAGTGCATTC |
| Sequence-based reagent | PPO1 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP002825 |
F:GACTCTACCCGGATCGGAAG R:ACTACCGTGATCGACTGGAC |
| Sequence-based reagent | PPO2 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP006258 |
F:TTGCGATGGTGACCGATTTC R:CGACGGTCCGGATACTTCTT |
| Sequence-based reagent | PPO3 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP004975 |
F:CTATTCGCCATGATCTCCAACTACG R:ATGACAGTGTTGGTGAAACGGATCT |
| Sequence-based reagent | PPO4 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP004981 |
F:GCTACATACACGATCCGGACAACTC R:CCACATCGTTAAATGCTAGCTCCTG |
| Sequence-based reagent | PPO5 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP012616 |
F:GTTCTCCTGTCGCTATCCGA R:CATTCGTCGCTTGAGCGTAT |
| Sequence-based reagent | PPO6 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP004977 |
F:GCAGCGGTCACAGATTGATT R:GCTCCGGTAGTGTTGTTCAC |
| Sequence-based reagent | PPO8 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP004976 |
F:CCTTTGGTAACGTGGAGCAG R:CTTCAAACCGCGAGACCATT |
| Sequence-based reagent | PPO9 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP004978 |
F:TGTATCCATCTCGGACGCAA R:AAGGTTGCCAACACGTTACC |
| Sequence-based reagent | rpS7 (qRT-PCR primers) | Kwon and Smith, 2019 | AGAP010592 |
F:ACCCCATCGAACACAAAGTTGACACT R:CTCCGATCTTTCACATTCCAGTAGCAC |
| Sequence-based reagent | SCRB3 (qRT-PCR primers) | This study | AGAP005725 |
F:CATCGGGACAGCTACATCCT R:TTATTGCTGCTACCGTTGCC |
| Sequence-based reagent | SCRB9 (qRT-PCR primers) | This study | AGAP004846 |
F:CGATATTCGGCGATGCAACT R:CACGCATGACACGATTCAGT |
| Sequence-based reagent | GFP (T7 RNAi primers) | Kwon and Smith, 2019 | NA |
F:TAATACGACTCACTATAGGGAGAATGGTGAGCAAGGGCGAGGAGCTGT R:CACGCATGACACGATTCAGT |
| Sequence-based reagent | Lz (T7 RNAi primers) | This study | AGAP002506 |
F:TAATACGACTCACTATAGGGCTGCAACCGTCCCAGAACAACGGC R:TAATACGACTCACTATAGGGACAAACCGGAGATCGTTGAATTTGG |
| Sequence-based reagent | Nimrod B2 (RNA-FISH probe) | Advanced Cell Diagnostics | AGAP029054 | Severo et al., 2018 |
| Sequence-based reagent | LRIM15 (RNA-FISH probe) | Advanced Cell Diagnostics | AGAP007045 | regions 2–874 of XM_308718.4 |
| Sequence-based reagent | Lz (RNA-FISH probe) | Advanced Cell Diagnostics | AGAP002506 | regions 168–1372 of XM_312433.5 |
| Sequence-based reagent | SCRB3 (RNA-FISH probe) | Advanced Cell Diagnostics | AGAP005725 | regions 337–1276 of XM_315741.5 |
| Sequence-based reagent | SCRB9(RNA-FISH probe) | Advanced Cell Diagnostics | AGAP004846 | regions 402–1306 of XM_001688510.1 |
| Commercial assay or kit | Standard macrophage depletion kit | Encapsula NanoSciences LLC | CLD-8901 | Control liposomes or clodronate liposomes were used in a 1:5 dilution in 1x PBS |
| Commercial assay or kit | DNA Clean and Concentration kit | Zymo Research | D4013 | |
| Commercial assay or kit | MEGAscript RNAi kit | Life Technologies | AM1626 | |
| Commercial assay or kit | RevertAid First Strand cDNA Synthesis kit | Life Technologies | K1622 | |
| Commercial assay or kit | RNAscope Multiplex Fluorescent Detection Reagents V2 |
Advanced Cell Diagnostics | 323110 | |
| Software, algorithm | Seurat | Butler et al., 2018 | ||
| Software, algorithm | Monocle3 | Cao et al., 2019 | ||
| Software, algorithm | alona | Franzén and Björkegren, 2020 |
https://alona.panglaodb.se/
https://github.com/oscar-franzen/alona/ |
|
| Software, algorithm | Graph Pad Prism | Graph Pad Software, LLC | ||
| Other | FITC-conjugatedWheat Germ Agglutinin (WGA) | Sigma | L4985 | 1:5000 |
| Other | DRAQ5 | Thermo Fisher Scientific | 62251 | 1:1000 |
| Other | Live/Dead Fixable Dead Cell Stain | Thermo Fisher Scientific | L34965 | 1:1000 |
| Other | FluoSpheres Fluorescent Microspheres | Molecular Probes | F8821, F8823 | Red or Green fluorescent fluorospheres for phagocytosis assays |
| Other | Opal Fluorophore reagent | Akoya Biosciences | Opal520 (FP1487001KT), Opal570 (FP1488001KT) | 1:1000 |
| Other | ProLongDiamond Antifade Mountant with DAPI | Life Technologies | P36966 | |
| Other | PowerUp SYBR Green Master Mix | Applied Biosystems | A25742 | |
| Other | E-RNAi | http://www.dkfz.de/signaling/e-rnai3/idseq.php | ||
| Other | DRSC RNA Seq Explorer | Tattikota et al., 2020 | https://www.flyrnai.org/scRNA/blood/ | |
| Other | Raddi et al., 2020 | https://hemocytes.cellgeni.sanger.ac.uk/ | ||
| Other | This study | https://alona.panglaodb.se/results.html?job=2c2r1NM5Zl2qcW44RSrjkHf3Oyv51y_5f09d74b770c9 |