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. 2021 Aug 5;9:681529. doi: 10.3389/fcell.2021.681529

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Copyright © 2021 Cao, Li, Li, Hu, Deng, Huang, Yang, Li and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TMEM220-AS1 inhibits the proliferation and cell cycle of HCC cells, but promotes the cell apoptosis of HCC cells. (A) Transfection efficiency of sh-TMEM220-AS1#1 (shRNA#1), sh-TMEM220-AS1#2 (shRNA#2) and TMEM220-AS1-overexpressing vector (LNC OE). (B) Cell viability was analyzed by (B) CCK-8 assay and (C) EdU assay (bar = 50 μm). (D) Cell cycle. (E) Cell apoptosis. Data were presented as represent the mean ± SD of 3 independent experiments; *P < 0.05, ** P < 0.01, ***P < 0.001.