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. 2021 Aug 5;9:681529. doi: 10.3389/fcell.2021.681529

TABLE 1.

qRT-PCR or shRNAs related sequences.

Name Sequence
Forward 5′-AGCTTCCACTCTTGTCTCCC-3′
Reverse 5′-TGAGCAGTGATGGAGCAGAA -3′
TMEM220-AS1
Forward 5′-ACACTCCAGCTGGGUAGCCCU CCCCUGACU-3′
Reverse 5′-CTCAACTGGTGTCGTGGAGTCG GCAATTCAGTTGAGAGTCCGAG-3′
miR-484
Forward 5′-GAACAAGGACCTGCGACATTT -3′
Reverse 5′-ACAGCATGGCGGTAAAGGTTA -3′
MAGI1
Forward 5′- GCTGGACCGAGAGAGTTTCC -3′
Reverse 5′- CAAAATCCAAGCCCGTGGTG -3′
E-cadherin
Forward 5′- CGGGAGAAATTGCAGGAGGA -3′
Reverse 5′-AAGGTCAAGACGTGCCAGAG-3′
Vimentin
Forward 5′-TCGGAAGCCTAACTACAGCGA-3′
Reverse 5′-AGATGAGCATTGGCAGCGAG-3′
Snail
Forward 5′- GATCCCCTGTAATCCCAGCTACT CAGCTTCCTGTCACTGAGTAGCTGG GATTACATTTTTGGAAA-3′
Reverse 5′- AGCTTTTCCAAAAATGTAATCCCA GCTACTCAGTGACAGGAAGCTGAGT AGCTGGGATTACAGGG-3′
TMEM220-AS1 shRNA #1
Forward 5′- GATCCCCTGGTGAAACCCCGTA TCTCCTTCCTGTCAGAGATACGGG GTTTCACCATTTTTGGAAA-3′
Reverse 5′- AGCTTTTCCAAAAATGGTGAAACC CCGTATCTCTGACAGGAAGGAGATAC GGGGTTTCACCAGGG-3′
TMEM220-AS1 shRNA #2