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. 2017 Mar 20;7(6):e2180. doi: 10.21769/BioProtoc.2180

Figure 3. rRNA N-glycosylase activity of BE27 in rabbit reticulocyte lysate.

Figure 3.

Each lane contained 3 µg of RNA isolated from either untreated (control) or RIP (+BE27) treated ribosomes from rabbit. The arrows indicate the 28S, 18S and 5.8S rRNAs, and the RNA fragment released as a result of RIP action after aniline treatment (+). The number of the corresponding tube in section A is indicated. Samples from lanes 5 and 6 were processed as samples from lanes 3 and 4, respectively, but the ribosomes were treated with 3 µg instead of 1 µg of BE27. The size of RNA markers in nucleotides are also indicated (M). The gel to the left was stained with ethidium bromide and the gel to the right with GelRed.