Table 1. Preparation of the 8% separation gel.

Reagent	Volume
ddH2O	3.47 ml
1.5 M Tris-HCl (pH 8.9)	1.88 ml
30% Acr-Bis	2.0 ml
10% SDS	75 µl
TEMED	4.5 µl
10% APS	75 µl
Total	7.5 ml