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. 2021 Aug 5;11(15):e4114. doi: 10.21769/BioProtoc.4114

Figure 1. The setup of the swim tunnel and water flow speeds.

Figure 1.

A. Different metal sheets can be used as the downstream screen to prevent exhausted fish from entering the water circulation. The two at the bottom (referred to as Metal Sheet 1 and Metal Sheet 2) are suitable for zebrafish studies. Pore diameter of Metal Sheet 1 is 0.5 cm and that of Metal Sheet 2 is 0.25 cm. B. The assembly of the metal sheet (indicated by the red arrow) in the swim tunnel. C. Water level (indicated by the red arrow) in the swim tunnel for all experiments. This is also the maximum capacity for this tunnel (about 40 L water). D. A picture showing where to place the air pump nozzle (indicated by the red arrow) in the tunnel. E. A picture showing where to place the flow meter to measure water speed (indicated by the red arrow). F. The linear correlation between water flow speed and revolutions per minute (RPM) of the motor when using either of the metal sheets shown in (A).